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The FASTQ input file size limit for free data analysis runs is 1.5 GB. Larger FASTQ file sizes may require additional quotas which can be purchased separately at sales@lexogen.com (Cat. No. 211 and 212 for QuantSeq FWD; Cat. No. 213 and 214 for QuantSeq REV). Alternatively, you can also try to compress your FASTQ files. For more information on how to achieve this, please this online FAQ My FASTQ files are bigger than 1.5GB what should I do?