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  • Leave approximately 2 µl of the eluate on the beads when removing the eluate for QC and sequencing. i.e., Transfer only 15 – 17 μl of the eluate into a fresh PCR plate or tube . and do not try to transfer the complete sample, as this will lead to bead carryover.

  • Put your samples once again on the magnet and incubate for 5 minutes.

  • Transfer 15-17 μl of the supernatant into a fresh PCR plate.Do not try to transfer the complete sample, as this will lead to bead carryover.

  • Put your samples once again on the magnet when taking an aliquot to run for QC on Tapestation/Bioanalyzer/Fragment Analyzer, or similar.