The kit includes a set of 96 i1 sample barcodes (i1 12 nt RT-Set for QuantSeq-Pool, dried-in sample barcodes in 96-well plate format), which allow up to 96 samples to be sequenced per lane on an Illumina flow cell. In this case, no i7 or i5 indices need to be added during the library amplification (endpoint PCR).
For multiplexing of more than 96 samples, or to combine multiple pools of smaller numbers of samples for the same sequencing run, unique dual indexing of individual pools (of up to 96 sample-barcoded libraries) is recommended.
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