Sample barcoding can be included in two ways, either during reverse transcription through the use of custom reverse transcription primers (see Appendix D of the TeloPrime V2 User Guide), or by adding sample barcodes after full-length cDNA amplification is complete. Sample multiplexing is supported for long-read sequencing platforms as well:
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FP-ONT: 5′– TTTCTGTTGGTGCTGATATTGC TGGATTGATATGTAATACGACTCACTATAG –3'
RP-ONT: 5′– ACTTGCCTGTCGCTCTATCTTC TCTCAGGCGTTTTTTTTTTTTTTTTTT — 3'
*bold sequence is the standard FP and RP sequence and the 5' sequence is the ONT primer binding sequence.
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