The qPCR assay requires the PCR Add-on and Reamplification Kit (Cat. no. 018), SYBR Green I dye (final concentration of 0.1x in the reaction mix) , and a real-time PCR machine. The PCR Add-on and Reamplification Kit (Cat. no. 018),
Determine the cycle number at which the fluorescence reaches at 80% of the maximum. If using the same volume of cDNA for the endpoint PCR, this is the cycle number to be used for the endpoint PCR.
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