TeloPrime is compatible with library preparation protocols for analysis on Pacific Biosciences and Oxford Nanopore Technologies platforms.
TeloPrime V2 and V1 libraries have been successfully sequenced on PacBio Sequel® instruments. For further details see our TeloPrime Publications page, or contact support@lexogen.com.
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Use 1 – 2 µg of total RNA as input for TeloPrime cDNA generation.
Use the highest possible quality of RNA.
Purify the amplified TeloPrime cDNA after the endpoint PCR using Purification Beads recommended in the respective protocol from Pacific Biosciences (for further details see also Appendix D of the TeloPrime V2 User Guide).
TeloPrime V2 Iso-Seq libraries are best loaded for Sequel instruments using the diffusion loading method. Loading amounts around 6 pM have produced high outputs for TeloPrime V2 libraries. However, specific loading amounts may need to be adjusted according to the instrument and chemistry version in use.
Contact Pacific Biosciences for further advice regarding the Iso-Seq™ protocols and sequencing recommendations.
TeloPrime libraries have been successfully sequenced on MinION devices and are featured in several recent publications.
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