Choose one or more compatible UDI Sets depending on the number of samples to be multiplexed and the Illumina instrument (using Forward Strand or Reverse Complement Workflow for dual-indexing) where the libraries will be sequenced. See Table 1 for details.
Choose the right product (Primer Plate, Add-on Kit or Bundle) based on the Library Prep Kit (Lexogen or other vendors). See Table 2 for a list of Library Prep Kits and the appropriate UDI product (with Catalog Number) to be indexed with, and see Table 3 for a list of our Library Prep Kits bundled with UDIs.
Choosing the correct UDI Set
The Lexogen UDI 12 nt Unique Dual Indexing Sets are available in different sets (A1-A4 and B1) for optimal compatibility with different Illumina sequencing instruments.
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6 These instruments use the reverse complement workflow, but the i5rc sequence is required to be entered if the analysis options available on the instrument (BaseSpace or Onboard instrument Dragen analysis) are used to generate a sample sheet. You will always be able to re-demultiplex with an updated sample sheet if modifications are required. Please review this article for a table of which instruments and softwares require which i5 index orientation or contact Illumina for additional support.
Unique Dual Indexing Product Offerings & Compatibility
There are three Lexogen UDI 12 nt Unique Dual Indexing product categories:
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Library Prep Type |
Base Kit(s) | UDI Products | |
UDI Primer Plate Only3 | UDI Add-on Kits(Primer Plate, Buffer, and Enzyme) | ||
QuantSeq-Pool1 | Cat. No. 139 | Cat. No. 101: Set A1 Cat. No. 102: Set A2 Cat. No. 103: Set A3 Cat. No. 104: Set A4 Cat. No. 156: Set A1 - A4 Cat. No. 105: Set B1 |
|
LUTHORHD | Cat. No. 204
| Cat. No. 101: Set A1 Cat. No. 102: Set A2 Cat. No. 103: Set A3 Cat. No. 104: Set A4 Cat. No. 156: Set A1 – A4 Cat. No. 105: Set B1 |
|
LUTHOR HD Pool1 | Cat. No. 205 | Cat. No. 101: Set A1 Cat. No. 102: Set A2 Cat. No. 103: Set A3 Cat. No. 104: Set A4 Cat. No. 156: Set A1 – A4 Cat. No. 105: Set B1 |
|
Other Vendor Libraries2 | N/A |
| Cat. No. 198: Set A1 Cat. No. 199: Set A2 Cat. No. 200: Set A3 Cat. No. 201: Set A4 Cat. No. 203: Set A1 - A4 Cat. No. 202: Set B1 |
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2 Libraries (RNA-Seq and DNA-Seq) from other vendors must contain TruSeq™ – compatible stubby adapters (where partial Illumina adapters are introduced during the workflow and completed with the index information during the endpoint PCR step). When unsure about compatibility please contact support@lexogen.com.
3 Each individual Set contains 96 UDIs.
Table 3: Lexogen Library Prep Kits including 12 nt UDIs (UDI Kit Bundles)
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