The LUTHOR single cell dispenser is primarily designed for usage with 96-well plates.
It is also possible to use it with 8-tube strips, 12-tube strips, and 384-well plates. In this case, please reach contact our technical support team at support@lexogen.com.
For 96-well plates, you can use non-skirted, semi-skirted, and full-skirted plates, with both standard or low profiles.
Non-skirted and semi-skirted plates will require a specific adapter , which is provided with the instrument and . The adapter is to be positioned onto the metal frame of the instrument.
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The software will offer you two programs: one for fully-skirted plates, and a second one for non-skirted and half-skirted plates.
IMPORTANT: Well bottom of the plates should be round shaped or conical, and not flat. This is due to the low volume (5 µl) of lysis buffer used to collect the cells during dispensing.
Any plate meeting ANSI/SLAS 1-2004 (footprint) and 4-2004 (well position) standards will be compatible with the LUTHOR single cell dispenser. For more information about these standards: https://www.slas.org/education/ansi-slas-microplate-standards/.
Aside from their format, it is highly recommended that plates are certified to be:
free Free of DNA and RNA
DNase-free and RNase-free
Low binding (if disclosed by manufacturer): low binding
NOTE: Similar recommendations also apply to for the pipette tips.