The Particle Threshold corresponds to the minimum impedance signal defined for a cell which helps to distinguish particles of interest from debris and other non-relevant particles.Below this value, particles are not detected during dispensing. A default threshold of 200 ohms is set-up in the analysis software (threshold defined for mammalian cells >10µm). This value can of course be adjusted depending on the experimental conditions (cell line and medium used).
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Additionally, thresholds can also be adjusted based on the sample distribution (see FAQ: How do I evaluate the concentration of my sample ?).
The distribution of the sample should follow a gaussian curve. Events detected before or after the curve can be considered as debris or maybe non-relevant particles.
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