Before dispensing, evaluation of particle size distribution of the sample can be done to determine the sample concentration and to estimate the particle and aggregate thresholds.
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Tcc corresponds to the time between two events. For Tcc is >5, the time between two events is long and the sample too diluted. In contrary, for Tcc <2, the sample is too concentrated.
IMPORTANT: if the sample is too concentrated, the accurate dispensing of individual cells might be altered! Unfortunately, it is not possible to dilute the sample and a new samples must be prepared! Please see the following FAQ: What are the cell sample concentration requirements? for an optimal preparation of your sample.
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The sample distribution displays a histogram which reveals the particles sizes following a gaussian curve. These results can be used to define the Particle and Aggregate thresholds (for more information about thresholds, please see FAQ: What are the “Particle” and “Aggregate” thresholds? How do I define them?).
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Figure | Electrical signal distribution of a sample. The “particle threshold” was set at 450 ohms.