The Particle Threshold corresponds to the minimum impedance signal defined for a cell which helps to distinguish particles of interest from debris and other non-relevant particles.Below this value, particles are not detected during dispensing. A default threshold of 200 ohms is set-up in the analysis software (threshold defined for mammalian cells >10µm). This value can of course be adjusted depending on the experimental conditions (cell line and medium used).
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The signal detected before the gaussian curve (450 ohm) can be defined as the particle threshold . The signal above 4000 ohms, in contrary can be defined as the aggregate threshold since the events are rare and outside of the gaussian curve.
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