For most applications single-read 50 or 75 (SR50, SR75) is sufficient for QuantSeq FWD sequencing.
When UMIs are included in QuantSeq FWD libraries, the minimum recommended sequencing length is SR75).
To increase the number of uniquely mapping reads or to better resolve exact 3' ends by reading into the poly(A) tail, longer reads may be used (SR100, SR150).
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