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  • Read 1: >=50bp. The pipeline will most likely also work for shorter read lengths. However, we should not advise the customer to use <50bp R1do not recommend using read lengths <50 bp for QuantSeq-Pool.

  • Read 2: >=22bp. The pipeline will also work for reads >22bp. However, after extracting the i1 and UMI from the first 22bp of R2, the remainder of R2 will be discarded . Hence, >22bp R2 cannot be used with our pipeline to perform a paired-end QuantSeq-Pool experimentand are therefore not recommended.