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The free BlueBee Data Analysis runs included with QuantSeq FWD/REV kits are limited to FASTQ files with a maximum size of 1.5 GB. If you have larger files you have two options:
Option 1
You can compress your FASTQ files and/or sub-sample the reads to reduce the size of your FASTQ files to 1.5 GB or less.
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Check out this Subsampling a fastq file with awk a command line tutorial.
Option 2
You can purchase Activation Codes for larger file sizes. Here are the catalog numbers you need:
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