The QuantSeq protocol is protocols are optimized for shorter reads (SR50 SR75 – 100) and yields yield mean library sizes of about 335 – 456 bp, with mean insert sizes of 203 – 324 bp.
To generate longer libraries, use the QuantSeq-Flex First Strand Synthesis Module V2 (Cat. No. 166) or contact support@lexogen.com for more information.