The recommended input range is 1 - 500 ng of total RNA.
No prior poly(A) enrichment or rRNA depletion of total RNA is required.
Low quality RNA including formalin-fixed parrafin embedded (FFPE) samples can also be used for QuantSeq.
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As a starting point, we recommend performing the protocol initially with 500 ng total RNA. RNA inputs ≥200 ng are recommended to detect low abundant transcripts efficiently.
NOTE! Input amounts <1 ng can also work, please consult the QuantSeq User Guides (015UG009, and 113UG227) for further details, or contact support@lexogen.com.