The recommended input range is 1 - 500 ng of total RNA. Protocol modifications apply for input amounts below 10 ng of total RNA, see: What is the minimum input amount for QuantSeq FWD? Are there any protocol modifications for low input amounts?
No prior poly(A) enrichment or rRNA depletion of total RNA is required.
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As a starting point, we recommend performing the protocol initially with 500 ng total RNA. RNA inputs ≥200 ng are recommended to detect low abundant transcripts efficiently.
NOTE! Input amounts <1 ng can also work, please consult the QuantSeq User Guides (015UG009, and 113UG227) for further details, or contact support@lexogen.com.