No, QuantSeq-Pool uses early pooling and thus equal read distribution in sequencing experiments requires normalization of the input RNA amount prior to starting library generation. This can be achieved best with high quality RNA (RIN > 6) with at least 10 ng per sample. The RNA input should be homogenous in terms of integrity, purity, and quantity across all samples. For further information please refer to the Appendix B in the QuantSeq-Pool Sample-Barcoded 3' mRNA-Seq User Guide.
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