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The QuantSeq 3' mRNA-Seq kits contain enough components to generate up to 96 reactions for manual or automated library preparation. Therefore, the provided reagent volumes should be sufficient to run all reactions at once on your liquid handler. However, if you want to split your kit into several machine runs you might loose a few reactions due to the higher dead volume needed compared to the manual preparation. Please contact us via support@lexogen.com if you experience reagent shortages.