Lexogen UDI 12 nt Sets contain a unique nested feature enabling adjustable index read-out lengths (the longer the index read-out, the better the error correction). The UDIs are 12 nucleotides long, however, the superior error correction feature can also be used if only 8 or 10 nucleotides of the index are read out, if the i5 index was read out in the correct orientation see Figure 1 and also FAQ 11.
[Quality]
Figure 1 | Optional read-out lengths for Lexogen UDI 12 nt SetsIf your library yields are extremely low and insufficient for pooling, reamplification can be performed using the Reamplification Add-on Kit for Illumina (080.96). This kit is available only upon request. Please contact Lexogen at support@lexogen.com for more information.
Please note that the PCR Add-on Kit (Cat. No. 020) cannot be used for reamplification of dual-indexed libraries.