Demultiplexing can be carried out by the standard Illumina pipelinetools (BaseSpace Sequence Hub and bcl2fastq2). Index sequences (UDI12A_0001-0384 and UDI12B_0001-0096) are available for download at www.lexogen.com/docs/indexing.
Additionally to the standard error-correction included in the standard Illumina pipelinetools, Lexogen’s idemuxCPP tool can be used to demultiplex and/or error correction. This command line tool is available free of charge at https://github.com/Lexogen-Tools/idemuxcpp.
Lexogen’s idemuxCPP can be used for:
i1 barcode demultiplexing of QuantSeq-Pool libraries
i5 and i7 demultiplexing of QuantSeq, QuantSeq-Pool, CORALL, and LUTHOR libraries
AND Error correction of above mentioned demultiplexed reads
Please contact support@lexogen.com for more information.