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The design of the advanced Lexogen UDI 12 nt Unique Dual Indexing system is not solely based on hamming distance, which only takes nucleotide substitutions into account, but is built on a global distance measure that accounts for substitutions, insertions, and deletions. This improves the error correction capability for the 12 nt UDIs using Lexogen’s iDemux idemuxCPP Tool for demultiplexing and error correction. For a set of 96 samples with 12 nt UDI read-out, the distance is 5, which allows for confident error correction of up to 2 index sequence errors. This is unique to Lexogen’s patented 12 nt UDI indexing system.
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