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The table below shows Endpoint PCR cycle numbers for Universal Human Reference RNA (UHRR) input amounts. This should be taken as a guideline only! Optimal cycle numbers could exceed these ranges depending on the sample type (i.e., species, tissue, RNA quality e.g., FFPE RNA, etc.). 
ATTENTION! :These cycle numbers are provided as a reference only and will not suit all sample types!
qPCR Assay for Endpoint PCR Cycle Number Determination
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The PCR Add-on Kit for Illumina (Cat. No. 020.96), and
SYBR Green I nucleic acid stain (10,000X in DMSO, not provided in the kit. We recommend using Sigma-Aldrich, Cat. No. S9430 or ThermoFisher, Cat. No. S7585)
ATTENTION: !The use of SYBR Green I-containing qPCR mastermixes from other vendors is not recommended.
Protocol
The recommended protocol for the qPCR assay is provided in the PCR Add-on Kit Instruction Manual, as well as in the Appendices of each QuantSeq 3' mRNA-Seq Library Prep Kit User Guide.
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