Tagging on the 5' end is based on Lexogen´s proprietary Cap Dependent Linker Ligation (CDLL) technology. After reverse transcription and generation of a double-stranded RNA : cDNA hybrid, an adapter with a 5' C overhang facilitates atypical base-pairing with the inverted G of the cap structure. Ligation takes place using a double-strand specific ligase, by ligating the 3' end of the double-stranded Cap linker to the 3' end of the cDNA.
Tagging of the 3' end of the mRNA takes place during the reverse transcription through the use of oligo(dT) priming.
Page Comparison
Manage space
Manage content
Integrations