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Libraries may also appear different depending on the microfluidics platforms used for quality control e.g., in TapeStation traces, the shape of the electropherogram curves appears shorter than in Bioanalyzer traces from the same libraries (see Figure, below).
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Figure : | Bioanalyzer and TapeStation traces from the same libraries. Library 1: 8x 10 ng UHRR per Second Strand Synthesis (SSS) reaction, 14 cycles, Library 2: 88x 10 ng Human Neural Progenitor Cells (hNPC) RNA per SSS, 11 cycles. Both libraries were run in a Bioanalyzer High Sensitivity DNA Chip (left plots) and in a TapeStation High Sensitivity D5000 ScreenTape (right graphs). Average Size was calculated from 170 – 3500 bp using the region analysis feature from the software specific to each microfluidics device.
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