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Globin Block libraries can also display: a longer library size distribution, and/or, peaks present at larger molecular weights (~400 bp and higher).
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Figure 1 : | Bioanalyzer traces for human QuantSeq FWD libraries prepared with (+Globin Block) and without (Standard) the RS-Globin Block solution (RS-GBHs, Cat. No. 070.96). Replicate libraries were prepared from 50 ng of whole blood (WB) RNA with the Standard QuantSeq FWD protocol (blue and red traces), versus QuantSeq +Globin Block (green and turquoise traces). RNA was isolated using the SPLIT RNA Extraction Kit without red blood cell lysis (Lexogen). Grey arrows indicate major globin peaks reduced in +Globin Block Libraries.
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