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The updated kits are provided with an a revised QuantSeq-Pool User Guide (139UG272V0300), which is already available online.
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The final reaction volumes and composition of the first strand cDNA synthesis reaction do not change due to separate addition of DTT.
The reduced holding temperature at Step 2 and omission of mastermix pre-warming has no negative impacts on library preparation or data quality.
There is no negative impact on data quality if libraries were already prepared with kits purchased prior to November 2021.
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