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• Samples with high DNA / RNA content (e.g., blood samples).

• Low quality / FFPE samples.

• Samples containing large quantities of short, extra-chromosomal DNA.

• Samples that have undergone mechanical disruption (gDNA fragmentation can increase carry-over potential)

What is the effect of gDNA contamination on my data?

The presence of gDNA contamination can strongly impact the sequencing data and lead to biases and quantification issues during data analysis.
A typical signature of gDNA contamination includes:

• A high proportion of intronic and intergenic reads and

• Loss of strand specificity (i.e., reads mapping to both strands)

For more information on gDNA contamination and DNAse treatment, please, check also our RNA LEXICON Chapter #5.