Choose one or more compatible UDI Sets depending on the number of samples to be multiplexed and the Illumina instrument (using Forward Strand or Reverse Complement Workflow for dual-indexing) where the libraries will be sequenced. See Table 1 for details.
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There are three Lexogen UDI 12 nt Unique Dual Indexing product categories:
UDI Primer Plate Only (amplification reagents not included)
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UDI Add-on Kit (primer plate + amplification reagents)
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Bundles (library prep kit + primer plate + amplification reagents)
NOTE: If UDIs should be included in libraries we recommend purchasing the Kit Bundles for ease of use.
Table Table 2: Lexogen UDI 12 nt Unique Dual Indexing product compatibility with Library Prep Kits.
Library Prep Type | Base Kit(s)with 6 nt indices or no indices included. | UDI Products | ||
UDI Primer Plate Only | UDI Add-onKitKits(Primer Plate, Buffer, and Enzyme) | BundleBundles(Library Prep Kit + UDI Primer Plate, Buffer, and Enzyme) | ||
QuantSeq-Pool1 | Cat. No. 139 | Cat. No. 101: Set A1 Cat. No. 102: Set A2 Cat. No. 103: Set A3 Cat. No. 104: Set A4 Cat. No. 156: Set A1 - A4 Cat. No. 105: Set B1 | ||
LUTHOR3,2 | Cat. No. 143 | Cat. No. 101: Set A1 Cat. No. 102: Set A2 Cat. No. 103: Set A3 Cat. No. 104: Set A4 Cat. No. 156: Set A1 – A4 Cat. No. 105: Set B1 | ||
QuantSeq FWD3 | Cat. No. 015 | Cat. No. 107: Set A1 Cat. No. 108: Set A2 Cat. No. 109: Set A3 Cat. No. 110: Set A4 Cat. No. 120: Set A1 - A4 Cat. No. 111: Set B1 | Cat. No. 113 – 115, 129– 131 | |
QuantSeq-REV3 | Cat. No. 016 | Cat. No. 107: Set A1 Cat. No. 108: Set A2 Cat. No. 109: Set A3 Cat. No. 110: Set A4 Cat. No. 120: Set A1 – A4 Cat. No. 111: Set B1 | ||
QuantSeq-Flex3 | Cat. No. 015 AND Cat. No. 166 AND/OR Cat. No. 028 | Cat. No. 107: Set A1 Cat. No. 108: Set A2 Cat. No. 109: Set A3 Cat. No. 110: Set A4 Cat. No. 120: Set A1 - A4 Cat. No. 111: Set B1 | ||
CORALL V2 | NA | Cat. No. 171 – 175 - stand alone kits Cat. No. 177 - 182 -with Poly(A) RNA Enrichment Cat. No. 183 - 186 - with RiboCop rRNA Depletion | ||
CORALL V1 | Cat. No. 095 (stand alone kit) OR Cat. No 144 - 146 (RiboCop bundles) | Cat. No. 107: Set A1 Cat. No. 108: Set A2 Cat. No. 109: Set A3 Cat. No. 110: Set A4 Cat. No. 120: Set A1 - A4 Cat. No. 111: Set B1 | Cat. No. 117 - 119, 132 - 134 - stand alone kits Cat. No. 158 - 163 - with Poly(A) RNA Enrichment | |
Other Vendor Libraries4 | NA | Cat. No. 107: Set A1 Cat. No. 108: Set A2 Cat. No. 109: Set A3 Cat. No. 110: Set A4 Cat. No. 120: Set A1 - A4 Cat. No. 111: Set B1 |
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3 The amplification reagents which are provided in the CORALL V1; QuantSeq -FWD, REV, and QuantSeq -Flex kits have to be replace replaced by the amplification reagents provided in the UDI Add-on kits (as instructed in the UDI Add-on Kit Instruction Manual).
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