Choose one or more compatible UDI Sets depending on the number of samples to be multiplexed and the Illumina instrument (using Forward Strand or Reverse Complement Workflow for dual-indexing) where the libraries will be sequenced. See Table 1 for details.
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Table 1: Lexogen UDI 12 nt Set selection table based on Illumina Instrument and number of samples.
Number of Samples | Illumina Instrument | |
|---|---|---|
Forward Strand Workflow (A) | Reverse Complement Workflow (B) | |
HiSeq 2000/2500 HiSeq 3000/4000 (Single-Read Flow Cell) MiSeq MiniSeq (Rapid Reagent Kits) NovaSeq 6000 (v1.0 Reagent Kits) | iSeq 100 HiSeq 3000/4000 (Paired-End Flow Cell) NextSeq 500 - 2000 MiniSeq (Standard Reagent Kit) NovaSeq 6000 (v1.5 Reagent Kits) HiSeq X | |
4 - 96 | Set A1 | Set B1 |
97 - 384 | Set A1 - A4 | Set A1 - A41,2 |
385 - 768 | 2x (Set A1 - A4)3 | 2x (Set A1 - A4)1,2,3 |
768 - 1152 | 3x (Set A1 - A4)4 | 3x (Set A1 - A4)1,2,4 |
1153 - 1536 | 4x (Set A1 - A4)5 | 4x (Set A1 - A4)1,2,5 |
1 When sequencing libraries indexed with UDI Sets A1-A4 on Illumina instruments that use the Reverse Complement Workflow (and vice versa for Set B1 UDIs used for Forward Strand Workflow), the i5 index sequences must be entered in the reverse complement orientation on the sample sheet for demultiplexing with Illumina demultiplexing tools (e.g., BaseSpace Sequence Hub or Bcl2fastq). EXAMPLE: i512A_0002 should be entered as TTAGTAACTGGG instead of CCCAGTTACTAA in the sample sheet for demultiplexing. The reverse complement sequences (i5rc) are provided the index sequence sheet available for download at www.lexogen.com/docs/indexing.
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UDI Primer Plate Only (amplification reagents not included)
UDI Add-on Kits (primer plate + amplification reagents)
UDI Kit Bundles (library prep kit + primer plate + amplification reagents)
NOTE: The UDI Add-on Kits can be used in combination with Base Kits that contain no indices, or that contain 6 nt i7 indices. When using the Base Kits indicated below one of the UDI Primer Plates or Add-on Kits will be needed in addition. However, if UDIs should be included in libraries we recommend purchasing the Kit Bundles for ease of use.
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