Choose one or more compatible UDI Sets depending on the number of samples to be multiplexed and the Illumina instrument (using Forward Strand or Reverse Complement Workflow for dual-indexing) where the libraries will be sequenced. See Table 1 for details.
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Table 1: Lexogen UDI 12 nt Set selection table based on Illumina Instrument and number of samples.
Number of Samples | Illumina Instrument | |
|---|---|---|
Forward Strand Workflow (A) | Reverse Complement Workflow (B) | |
HiSeq 2000/2500 HiSeq 3000/4000 (Single-Read Flow Cell) MiSeq MiniSeq (Rapid Reagent Kits) NovaSeq 6000 (v1.0 Reagent Kits) | iSeq 100 HiSeq 3000/4000 (Paired-End Flow Cell) NextSeq 500 - 2000 MiniSeq (Standard Reagent Kit) NovaSeq 6000 (v1.5 Reagent Kits) HiSeq X | |
4 - 96 | Set A1 | Set B1 |
97 - 384 | Set A1 - A4 | Set A1 - A41,2 |
385 - 768 | 2x (Set A1 - A4)3 | 2x (Set A1 - A4)1,2,3 |
768 - 1152 | 3x (Set A1 - A4)4 | 3x (Set A1 - A4)1,2,4 |
1153 - 1536 | 4x (Set A1 - A4)5 | 4x (Set A1 - A4)1,2,5 |
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UDI Primer Plate Only (amplification reagents not included)
UDI Add-on Kits (primer plate + amplification reagents)
UDI Kit Bundles (library prep kit + primer plate + amplification reagents)
NOTE: The UDI Add-on Kits can be used in combination with Base Kits that contain no indices, or that contain 6 nt i7 indices. When using the Base Kits indicated below one of the UDI Primer Plates or Add-on Kits will be needed in addition. However, if UDIs should be included in libraries we recommend purchasing the Kit Bundles for ease of use.
Table 2: Lexogen UDI 12 nt Unique Dual Indexing product compatibility with Library Prep Kits.
Library Prep Type | Base Kit(s)with 6 nt indices or no indices included. | UDI Products | ||
UDI Primer Plate Only | UDI Add-on Kits(Primer Plate, Buffer, and Enzyme) | UDI Kit Bundles(Library Prep Kit + UDI Primer Plate, Buffer, and Enzyme) | ||
QuantSeq-Pool1 | Cat. No. 139 | OPTIONALLY one of: Cat. No. 101: Set A1 Cat. No. 102: Set A2 Cat. No. 103: Set A3 Cat. No. 104: Set A4 Cat. No. 156: Set A1 - A4 Cat. No. 105: Set B1 | ||
LUTHOR2 | Cat. No. 143 | AND one of: Cat. No. 101: Set A1 Cat. No. 102: Set A2 Cat. No. 103: Set A3 Cat. No. 104: Set A4 Cat. No. 156: Set A1 – A4 Cat. No. 105: Set B1 | ||
QuantSeq FWD3 | Cat. No. 015 | AND one of: Cat. No. 107: Set A1 Cat. No. 108: Set A2 Cat. No. 109: Set A3 Cat. No. 110: Set A4 Cat. No. 120: Set A1 - A4 Cat. No. 111: Set B1 | OR one of the following UDI Kit Bundles: Cat. No. 113 – 115, 129 – 131 | |
QuantSeq-REV3 | Cat. No. 016 | AND one of: Cat. No. 107: Set A1 Cat. No. 108: Set A2 Cat. No. 109: Set A3 Cat. No. 110: Set A4 Cat. No. 120: Set A1 – A4 Cat. No. 111: Set B1 | ||
QuantSeq-Flex3 | Cat. No. 166 AND/OR Cat. No. 028 | AND one of the following QuantSeq FWD UDI Kit Bundles Cat. No. 113 – 115, 129 – 131 | ||
CORALL V2V2 | NA | Cat. No. 171 – 176 - stand alone kits Cat. No. 177 - 182 - with Poly(A) RNA Enrichment Cat. No. 183 - 186 - with RiboCop rRNA Depletion | ||
CORALL V1V1 | Cat. No. 095 (stand alone kit) OR Cat. No 144 - 146 (RiboCop bundles) | AND one of: Cat. No. 107: Set A1 Cat. No. 108: Set A2 Cat. No. 109: Set A3 Cat. No. 110: Set A4 Cat. No. 120: Set A1 - A4 Cat. No. 111: Set B1 | OR one of the following UDI Kit Bundles: Cat. No. 117 - 119, 132 - 134 - stand alone kits Cat. No. 158 - 163 - with Poly(A) RNA Enrichment | |
Other Vendor Libraries4 | NA | OPTIONALLY one of: Cat. No. 107: Set A1 Cat. No. 108: Set A2 Cat. No. 109: Set A3 Cat. No. 110: Set A4 Cat. No. 120: Set A1 - A4 Cat. No. 111: Set B1 | ||
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