The Lexogen UDI 12 nt Unique Dual Indexing V2 Add-on Kits are in theory compatible with all non-Lexogen library prep kits utilizing TruSeq™ – compatible stubby adapters . Where partial Illumina adapters are introduced during the workflow and completed with the index information during the endpoint PCR step(P5 and P7 adapters).
P5 and P7 can be either ligated to the cDNA insert or also introduced by PCR, depending on the manufacturer’s protocol.
Our UDI will be subsequently added by PCR (with the amplification mix provided in the Lexogen UDI Add-on V2 kit) on existing P5 and P7 stubby sequences.
Please contact support@lexogen.com for more information, or in case you have any doubts about potential compatibility issues between your library generation kit and our UDI Add-on module.