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The updated kits are provided with a revised User Guide (see Previous Version: 139UG272V0300) that is already available online.
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Step 2: Reactions are cooled to, then held at 25 °C instead of 42 °C after denaturing the RNA / RT Primer mix.
Step 3: The mastermix is prepared with FS + DTT + Enzyme Mix 1 (E1). Prewarming of this mastermix at 42 °C is no longer required and was removed from the protocol.
ATTENTION! FS from kits that do not contain the DTT component (i.e., kits purchased before December 2021) cannot be combined with DTT using the updated QuantSeq-Pool protocol (139UG272V0300). In case of any questions please contact support@lexogen.com.
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