No. QuantSeq FWD and REV libraries are prepared using the same protocol steps and reaction volumes.
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Therefore, the same automation protocol can be used for preparing all types of QuantSeq FWD and REV libraries, with or without Globin Block and / or UMIs.
NOTE:QuantSeq Flex libraries may require different automated protocol modifications depending on the use of custom primers at either or both of first and second strand synthesis steps of library generation. Please contact support@lexogen.com for more information.
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