Both kit versions yield sequences close to the 3' end of transcripts. The difference is in the location of the Read 1 linker sequence. If it is located in the 5' part of the second strand synthesis primer (QuantSeq FWD, Cat. No. 015, 113 – 115, 129 - 131, and 191 - 196), NGS reads will be generated towards the poly(A) tail.
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