Choose one or more compatible UDI Sets depending on the number of samples to be multiplexed and the Illumina instrument (using Forward Strand or Reverse Complement Workflow for dual-indexing) where the libraries will be sequenced. See Table 1 for details.
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UDI Primer Plate Only (amplification reagents not included)
UDI Add-on Kits (primer plate + amplification reagents)
UDI Kit Bundles (library prep kit + primer plate + amplification reagents)
NOTE: The UDI Add-on Kits can be used in combination with Base Kits that contain no indices or that contain 6 nt i7 indices. When using the Base Kits indicated below, one of the UDI Primer Plates or Add-on Kits will be needed in addition. However, if UDIs should be included in libraries, we recommend purchasing the Kit Bundles for ease of use.
Table 2: Lexogen UDI 12 nt Unique Dual Indexing product compatibility with Library Prep Kits.
Library Prep Type |
Base Kit(s)with 6 nt indices or no indices included. | UDI Products | ||
UDI Primer Plate Only | UDI Add-on Kits(Primer Plate, Buffer, and Enzyme) | UDI Kit Bundles(Library Prep Kit + UDI Primer Plate, Buffer, and Enzyme) | ||
QuantSeq-Pool1 | Cat. No. 139 | OPTIONALLY one of: Cat. No. 101: Set A1 Cat. No. 102: Set A2 Cat. No. 103: Set A3 Cat. No. 104: Set A4 Cat. No. 156: Set A1 - A4 Cat. No. 105: Set B1 | ||
LUTHOR2 | Cat. No. 143 | AND one of: Cat. No. 101: Set A1 Cat. No. 102: Set A2 Cat. No. 103: Set A3 Cat. No. 104: Set A4 Cat. No. 156: Set A1 – A4 Cat. No. 105: Set B1 | ||
QuantSeq FWD3 | Cat. No. 015 |
| AND one of: Cat. No. | |
198: Set A1 Cat. No. |
199: Set A2 Cat. No. |
200: Set A3 Cat. No. |
201: Set A4 Cat. No. |
203: Set A1 - A4 Cat. No. |
202: Set B1 | OR one of the following UDI Kit Bundles: Cat. No. |
191 - 196 | |||
QuantSeq-REV3
| Cat. No. 016 | AND one of: Cat. No. |
198: Set A1 Cat. No. |
199: Set A2 Cat. No. |
200: Set A3 Cat. No. |
201: Set A4 Cat. No. |
203: Set A1 |
- A4 Cat. No. |
202: Set B1 | ||||
QuantSeq-Flex3 | Cat. No. 166 AND/OR Cat. No. 028 |
| AND one of the following QuantSeq FWD UDI Kit Bundles Cat. No. |
CORALL V2
NA
Cat. No. 171 – 176 - stand alone kits
Cat. No. 177 - 182 - with Poly(A) RNA Enrichment
Cat. No. 183 - 186 - with RiboCop rRNA Depletion
191 - 196 | |||
CORALL | Cat. No. 095 (stand alone kit) OR Cat. No 144 - 146 (RiboCop bundles) |
| AND one of: Cat. No. |
198: Set A1 Cat. No. |
199: Set A2 Cat. No. |
200: Set A3 Cat. No. |
201: Set A4 Cat. No. |
203: Set A1 - A4 Cat. No. |
202: Set B1 | OR one of the following UDI Kit Bundles: Cat. No. |
171 – 176 - stand alone kits Cat. No. |
177 - |
182 - with Poly(A) RNA Enrichment Cat. No. 183 - 186 - with RiboCop rRNA Depletion | |||
Other Vendor Libraries4 | NA |
| OPTIONALLY one of: Cat. No. |
198: Set A1 Cat. No. |
199: Set A2 Cat. No. |
200: Set A3 Cat. No. |
201: Set A4 Cat. No. |
203: Set A1 - A4 Cat. No. |
202: Set B1 |
|
1 QuantSeq-Pool kits contain 96 in-line indices (i1) for individual sample barcoding before pooling. For analysis of more than 96 samples additional indexing with e.g., UDIs is required.
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