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Lexogen Data Analysis
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Solutions
Automated Lexogen offers data analysis solutions for QuantSeq FWD-UMI libraries is available on the BlueBee® Genomics Platform.
To Starting from January 2023, to analyze data from QuantSeq FWD libraries that contain UMIs, simply use the activation code included with in your QuantSeq FWD library prep kit and select the respective "FWD-UMI" pipeline when setting up your data analysis run.
Be sure to only select the "FWD-UMI" pipelines for UMI-containing libraries, otherwise duplicate reads will not be collapsed.
follow the instruction provided in this online FAQ for submitting your Data Analysis Request.
For further details see Can How can I analyze my QuantSeq data using Lexogen data analysis solutions?
For updates on the Data Analysis Pipelines on the BlueBee® Genomics Platform?data analysis solutions provided by Lexogen, please check this Webpage: Lexogen Data Analysis Solutions | Lexogen
UMI-Tools
As an alternative to BlueBeethe data analysis solutions offered by Lexogen, we recommend utilizing the publicly available UMI-Tools package available on GitHub here. Detailed documentation can be found in the ReadTheDocs. The following command line will extract the UMI sequence from the read while removing the adjacent 4 nt TATA spacer:
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The deduplication method of UMI-Tools has been published here.
NOTE: The current implementation of this method can take some time and can consume significant memory. If you experience issues with run time or memory usage, please refer to these FAQs.
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