No, currently the QuantSeq data analysis pipelines on BlueBee are only compatible with standard QuantSeq FWD and REV library data analysis. Lexogen offers a specific QuantSeq-Pool data analysis pipeline, which can be found on our GitHub page.
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QuantSeq Pool libraries include the Read 1 linker sequence in the 5' part of the second strand synthesis primer, hence NGS reads are generated towards the poly(A) tail and reflect the sequence of the corresponding mRNA.
The 10 nt UMI sequence and subsequent 12 nt sample barcodes must be read out in read 2, therefore paired-end reads are required as input for QuantSeq-Pool data anlaysisanalysis.