If your libraries are undercycled or the yields are insufficient for pooling and sequencing, you can re-amplify your libraries using the PCR Add-on and Reamplification Kit Kit V2.
For libraries with low but measurable yields, we recommend using 3-6 cycles for the library reamplification (approximatedoubling of the original yield per cycle).
For libraries with no measurable concentration and/or no visibletraces on microcapillary devices (e.g., Bioanalyzer), we recommend using 6-10 cycles for reamplification. For further PCR cycle recommendations for reamplification, please contact: support@lexogen.com.
NOTE: We recommend performing quality control of finished libraries using 1) high sensitivity microcapillary electrophoresis (e.g., Bioanalyzer) for assessing library size distribution/profile and 2) Qubit high sensitivity assay for quantification.