The main difference is the introduction of an updated PCR system in the new V2 kit compared to V1.The new PCR Add-on and Reamplification Kit V2 contains a new and improved PCR system.
In the V2 Kit, the PCR Mix “PM” and the PCR enzyme Mix “PE” replace the PCR Mix “PCR” and the Enzyme Mix “E” provided with the PCR Add-on Kit.
For the qPCR assay, in the V2 kit, both primers “P7” and “P5” are provided separately for a universal use of the kit.
For the library reamplification, the Reamplification primer Mix “RE” replaces the i7 reamplification Primer “i7-RE” included in the V1 kitKit.
NOTE: With the updated PCR system, a new PCR program has also been implemented. Please refer to the UserGuide p6 for the qPCR assay and p12 for the library reamplification for specific details.
IMPORTANT: The new PCR Add-on and Reamplification kit Kit V2 is not recommended for qPCR assays with older kit version versions of QuantSeq and CORALL products(Cat. No. 015, 016, 113 - 115, 129 - 131) and CORALL (Cat. No. 095, 116 - 119, 123 - 134). When using libraries prepared with previous versions of QuantSeq and CORALL library prep kits, please refer to the PCR Add-on kit Kit (Cat.No.020) for qPCR assay or endpoint PCR.