The new PCR Add-on and Reamplification Kit V2 (Cat. No. 208) contains combines the application purposes of the PCR Add-on Kit (Cat. No. 020) and the Reamplification Add-on Kit (Cat. No. 080) in one single kit and introduces a new and improved PCR system.
In the V2 Kit, the PCR Mix “PM” and the PCR enzyme Mix “PE” replace the PCR Mix “PCR” and the Enzyme Mix “E” provided with of the PCR Add-on Kit.
Additionally,both primers used for qPCR (“P7” and “P5”) are now provided separately, allowing for a universal use of the kit. For library reamplification, the V2 Reamplification primer Mix “RE” replaces the i7 reamplification Primer “i7-RE” included in the V1 Kit. primers necessary for the qPCR assay (“P7” and ”P5”) and the library reamplification (Reamplification Primer Mix ”RE”) are provided separately, allowing for a universal use of the kit.
NOTE: With the updated PCR system, a new PCR program has also been implemented. Please refer to the UserGuide User Guide p6 for the qPCR assay and p12 for the library reamplification for specific details.
IMPORTANT: For the qPCR assay, the new PCR Add-on and Reamplification Kit V2 is not recommended in combination with older versions (V1)of QuantSeq and CORALL (see table below). When using libraries prepared with previous versions, please refer to the PCR Add-on Kit (Cat.No.020).
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PCR Add-on and Reamplification Kit V2 (Cat. No. 208) | ||
|---|---|---|
Kit | qPCR assay | Reamplification |
QuantSeq V1 (Cat. No. 015, 016, 113 - 115, 129 - 131) | No | Yes |
CORALL V1 (Cat. No. 095, 116 - 119, 123 - 134) | No | Yes |
QuantSeq V2 (Cat. No. 191 - 196) | Yes | Yes |
CORALL V2 (Cat. No. 171 - 186) | Yes | Yes |
Compatibility of PCR Add-on Kit V1 (Cat. No. 020) with QuantSeq and CORALL V1 and V2.
PCR Add-on Kit V1 (Cat. No. 020) | ||
|---|---|---|
Kit | qPCR assay | Reamplification |
QuantSeq V1 (Cat. No. 015, 016, 113 - 115, 129 - 131) | Yes | Only with single indexing |
CORALL V1 (Cat. No. 095, 116 - 119, 123 - 134) | Yes | Only with single indexing |
QuantSeq V2 (Cat. No. 191 - 196) | Yes* | No |
CORALL V2 (Cat. No. 171 - 186) | Yes* | No |
*For these kits, the PCR Add-on and Reamplification Kit V2 (Cat. No. 208) is the optimal choice for qPCR.