OLD:
LUTHOR uses a unique technology to generate libraries. The libraries present with broad size distribution.
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Figure: Bioanalyzer traces of LUTHOR libraries prepared from different input amounts of purified total Universal Human Reference RNA (UHRR). Libraries were prepared using 10 pg (red trace, 11 PCR cycles), 25 pg (blue trace, 9 PCR cycles), 50 pg (green trace, 9 PCR cycles), and 100 pg (cyan trace, 8 PCR cycles) of UHRR input. Endpoint PCR was performed using i5 / i7 dual indexing.
NEW
LUTHOR HD uses a proprietary technology to generate libraries that present with varying size distributions. Library shape and average size may vary depending the the type of input sample (e.g., degraded, low quality RNA produces shorter library inserts than high quality RNA). The majority of inserts are greater than 100bp in size, corresponding to final library fragment sizes ≥ 300bp.
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