OLD
LUTHOR can be used for direct RNA amplification from animal cells or protoplasts (e.g., from plants), or with purified RNA from all organisms and viruses that possess poly(A)-tailed RNA species.
LUTHOR is not suitable for analysis of non-polyadenylated RNA, e.g., total RNA from bacterial species.
Input ranges:
FACS-Sorted Cells: 1 – 100 cells per well/sample may be used. For sorting of single cells, we recommend sorting directly into 5 µl of Cell Lysis Buffer (CLB).
Cell suspensions: 1 – 100 cells/sample in a volume of 2 µl. Prepare cell suspension in 1x PBS.
Purified RNA: 10 pg – 1 ng in a volume of 4 µl.
Higher inputs are possible and require minor protocol modifications. For more information, please contact Lexogen Tech Support at support@lexogen.com.
NEW
LUTHOR HD can be used for direct RNA amplification from animal cells or protoplasts (e.g., from plants) or with purified RNA from all organisms and viruses that possess polyadenylated poly(A)-tailed RNA species.
LUTHOR HD is not suitable for the analysis of non-polyadenylated RNA, e.g., total RNA from bacterial species.
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FACS-Sorted Cells: 1 – 100 cells per well/sample may be used.
For sorting of single cells, we recommend sorting directly into 5 µl of Cell Lysis Buffer (CLB).Cell suspensions:
1 or more.1 – 100 cells/sample. Prepare cell suspension in 1x PBS.Purified RNA: 10 pg – 1 ng in a volume of 3 µl.
Higher inputs are not recommended, and samples should be diluted to meet recommended input guidelines. For more information, please contact Lexogen Tech Support at support@lexogen.com.
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