The CORALL Data Analysis The CORALL data analysis pipeline includes:
UMI extraction
Trimming (Cutadapt V1.18)
FastQC analysis of trimmed FASTQ files (FASTQC V0.11.7)
Alignment (STAR V2.6.1a)
UMI collapsing (more info can be found here)
Mix² for transcript isoform quantification (Mix² is a Lexogen´s proprietary software
;, for more information,please visit our webpage https://www.lexogen.com/mix-analysis-software/?_gl=1*bg2il0*_ga*MjM1NzQ5NTYuMTY2MjEwNjEzMw..*_ga_C4BTKL3CGH*MTY4MjQzODYyNy42NzUuMS4xNjgyNDM4NzAxLjAuMC4w)Gene counting (FeatureCounts V1.6.4) > not sure
Differential gene expression analysis (DESeq2 V1.18.1)
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