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Should amounts of undercycled libraries be insufficient for sequencing, the PCR Add-on and Reamplification Kit for IlluminaV2 (Cat. No. 020208) includes a Reamplification Primer that can be used to add some PCR cycles for your undercycled libraries (QuantSeq 3' mRNA-Seq User Guide, Appendix D). Please note that currently only single indexed libraries can be reamplified using the PCR Add-on Kit (Cat. No. 020). Dual-indexed libraries can only be reamplified using the Reamplification Add-on Kit for Illumina (Cat. No. 080.96), which is available upon request by contacting support@lexogen.com. This Reamplification Add-on Kit can also be used to reamplify single-indexed librariesand Reamplification Kit V2 User Guide, Section 6.1).