The qPCR assay requires the addition of PCR Add-on and Reamplification Kit (Cat. no. 018), SYBR Green I dye to the PCR (final concentration of 0.1x in the reaction mix) and performing the amplification using , a real-time PCR machine.
Determine the cycle number at which the fluorescence reaches at 80% of the maximum. If using the same volume of cDNA for the endpoint PCR, this is the cycle number to be used for the endpoint PCR.
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