The loading guidelines provided below are for Lexogen libraries prepared using the Adept workflows for sequencing on the Element Bioscience AVITI sequencing platform. These guidelines are based on the current experience of Lexogen and Lexogen customers.
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Library Type | Recommended Read Format (Seq. Kit)* | Custom Primers Required? | Average Size Range | CloudBreak Circular Adept v1.1 Libraries | CloudBreak Linear Adept Rapid PCR-Plus Libraries |
|---|---|---|---|---|---|
CORALL RTM | SR150; PE150 (2x75; 2x150) | No | 300 - 450 bp | 6 - 6.5 pM2 2 - 5% PhiX | |
CORALL RTL | PE-150 (2x150) | No | 500 - 600 bp | 8 - 8.5 pM2 2 - 5% PhiX | |
QuantSeq FWD | SR150 (2x75)1 | No | 250 - 400 bp | 5 - 6 pM4 2% PhiX | 12 pM4 |
400 - 500 bp | 7 - 8 pM2,4 2% PhiX | ||||
QuantSeq FWD-UMI | SR150 (2x75)1 | No | 250 - 400 bp | 7.5 - 8.5 pM4 2% PhiX for runs with <= 20% of UMI libraries in total lanemix. -for advice on PhiX for runs that contain >20% QuantSeq FWD-UMI libraries please contact support@lexogen.com | |
QuantSeq-Pool | PE125/25 (2x75) | No | 400 - 550 bp | 8.5 - 10 pM4 2% PhiX | |
Luthor HD | PE125/25 (2x75) | No | 500 - 600 bp | 8.5 - 10 pM4 2% PhiX | |
Mixed Lexogen libraries (excluding small RNA-Seq) | SR150; PE75; PE150 (2x75, 2x150) | No | 300 - 400 bp | 6 pM4 2% PhiX | |
SR150; PE75; PE150 (2x75, 2x150) | No | 500 - 700 bp | 9 - 11 pM4 2% PhiX | ||
Lexogen small RNA-Seq | SR75; PE75 (2x75) | Yes [R1, R2, I2] | 143 - 200 bp | Please contact support@lexogen.com for updated recommendations if you wish to sequence these library types on the AVITI. | |
QuantSeq REV 3' mRNA Seq | SR150 (2x75)1 | No - if combined with other Lexogen libraries at <= 10% of lanemix.4 | 250 - 400 bp | ||
*The 2 x 300 cycle kits are not recommended for sequencing Lexogen library types.
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