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Learn more about UMIs, their function, and usage in our Lexicon Chapter #8.
The i7 (and i5) indices 12 nt Unique Dual Indexes (UDIs) are still required if multiple libraries need to be uniquely barcoded and pooled for sequencing in a single lane or run. The i7 and i5 index UDI sequences are added during the endpoint PCR and read-out during sequencing with index reads 1 and 2, respectively. The index reads are required for sample demultiplexing after sequencing.
The i7 indices UDIs required for the final amplification of QuantSeq libraries are included as a bundle in every QuantSeq Library Prep Kit . Alternatively, 12 nt Unique Dual Indices (UDIs) can be used to enable uniquely dual-indexed libraries for Illumina sequencing.QuantSeq Library Prep Kits and UDIs are available in bundle versions (Cat. No.s 191 - 196).