If your library yields are extremely low and insufficient for pooling, reamplification can be performed using the Reamplification Add-on Kit for Illumina (080.96). This kit is available only upon request. Please contact Lexogen at support@lexogen.com for more information.
Please note that the PCR Add-on Kit (Cat. No. 020) cannot be used for reamplification of dual-indexed librariesDemultiplexing can be carried out by the standard Illumina pipeline. Index sequences (UDI12A_0001-0384 and UDI12B_0001-0096) are available for download at www.lexogen.com/docs/indexing.
Additionally to the standard error-correction included in the Illumina pipeline, Lexogen’s idemuxCPP tool can be used to demultiplex and/or error correction. This command line tool is available free of charge at https://github.com/Lexogen-Tools/idemuxcpp.
Please contact support@lexogen.com for more information.