- Choose one or more compatible UDI Sets depending on the number of samples to be multiplexed and the Illumina instrument (using Forward Strand or Reverse Complement Workflow for dual-indexing) where the libraries will be sequenced. See Table 1 for details.
- Choose the right product (Primer Plate, Add-on Kit or Bundle) based on the Library Prep Kit (Lexogen or other vendors). See Table 2 for a list of Library Prep Kits and the appropriate UDI product (with Catalog Number) to be indexed with.
...
Library Prep Kit | UDI product |
|
|
| Primer Plate Only | Add-on Kit(Primer Plate, Buffer, Enzyme) | Bundle(Library Prep Kit + Primer Plate, Buffer and Enzyme) |
QuantSeq-Pool1Cat. No. 139 | Cat. No. 101: Set A1Cat. No. 102: Set A2Cat. No. 103: Set A3Cat. No. 104: Set A4Cat. No. 156: Set A1-A4Cat. No. 105: Set B1 |
|
|
LUTHOR 3' 2Cat. No. 143 | Cat. No. 101: Set A1Cat. No. 102: Set A2Cat. No. 103: Set A3Cat. No. 104: Set A4Cat. No. 156: Set A1-A4Cat. No. 105: Set B1 |
|
|
QuantSeq-Rev3Cat. No. 016 |
| Cat. No. 107: Set A1Cat. No. 108: Set A2Cat. No. 109: Set A3Cat. No. 110: Set A4Cat. No. 120: Set A1-A4Cat. No. 111: Set B1 |
|
QuantSeq-Flex3Cat. No. 033-035 |
| Cat. No. 107: Set A1 Cat. No. 108: Set A2Cat. No. 109: Set A3Cat. No. 110: Set A4Cat. No. 120: Set A1-A4Cat. No. 111: Set B1 |
|
Other Vendor Libraries4 |
| Cat. No. 107: Set A1Cat. No. 108: Set A2Cat. No. 109: Set A3Cat. No. 110: Set A4Cat. No. 120: Set A1-A4Cat. No. 111: Set B1 |
|
QuantSeq-FWDCat. No. 015 |
|
| Cat. No. 113-115, 129-131 |
CORALLCat. No. 095 |
|
| Cat. No. 117-119, 132-134 |
. Table 2 | Lexogen UDI 12 nt Unique Dual Indexing product compatibility with Library Prep Kits.
1 QuantSeq-Pool kits contain 96 inline barcodes (i1) for individual sample barcoding before pooling. For analysis of more than 96 samples additional indexing with e.g. UDIs is required.
2 LUTHOR kits do not include barcodes. Additional purchase is required for functionality of the library prep.
3 The amplification reagents which are provided in the QuantSeq-Rev and QuantSeq-Flex kits have to be replace by the amplification reagents provided in the UDI Add-on kits (as instructed in the UDI Add-on Kit Instruction Manual).
4 Libraries (RNA-Seq and DNA-Seq) from other vendors must contain TruSeq™ – compatible stubby adapters (where partial Illumina adapters are introduced during the workflow and completed with the index information during the endpoint PCR step). When unsure about compatibility please contact support@lexogen.comThe NovaSeq Xp workflow enables individual lane loading on the same flow cell. Hence the multiplexing capacity can be expanded 2 times using the NovaSeq XP 2-Lane Kit or 4 times with the NovaSeq XP 4-Lane Kit. Hence, it is possible load up to 4 physically separated 384-plex UDI-indexed libraries per flow cell. For more information about the Xp Workflow, please refer to the NovaSeq™ Xp workflow Technical Note, The NovaSeq™ Xp workflow explanatory video, or contact Illumina Support.